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KMID : 1094720120170030634
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Biotechnology and Bioprocess Engineering
2012 Volume.17 No. 3 p.634 ~ p.642
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Development of ERE/DRE-dual CALUX bioassays system for monitoring estrogen- and dioxin-like persistent organic pollutants
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Kwon Mi-Sun
Lee Hyo-Jin
Kim Sun-Hee
Lim Se-Young
Park Jong-Moon
Ryu Sung-Ho
Yang Jae-Ho
Suh Pann-Ghill
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Abstract
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Estrogen- and dioxin-like responsive element mediated Chemically Activated LUciferine gene eXpression (ERE/DRE-dual CALUX) was utilized to develop dualbioassays for simultaneously determining estrogen and dioxin levels. The devised ERE/DRE-dual CALUX bioassay system, was applied to the analysis of 103 estrogen and dioxin-related isomers, PAHs, PCBs, and pesticides. The nonylphenol highly induced estrogen-like activity (9.95 times) while benzo(k)fluoranthene, chrysene (zonerefined), and 3,3′,4,4′,5-pentachlorobiphenyl, highly induced dioxin-like activity (32.23, 42.39, and 34.47 times) respectively as compared with the untreated control. To confirm the validity of the devised ERE/DRE-dual CALUX bioassay system, we compared this bioassay with high-resolution gas chromatography/high-resolution mass spectrometry (HRGC/HRMS) to assess five air samples taken in Korea. The correlation coefficient between the ERE/DRE-dual CALUX bioassay system and HRGC/HRMS method showed a good relationship (r2 = 0.92). This study indicates that ERE/DRE-dual CALUX is a simple, high-throughput, valid, and inexpensive bioassay method for screening large numbers of environmental samples.
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KEYWORD
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dioxin, estrogen, CALUX, bioassays, HRGC/HRMS
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